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    视频

    与氟喹诺酮和氨基糖苷类对鸟分枝杆菌分离株耐药性有关的单核苷酸多态性

     

    Authors Pang H, Wan K, Wei L

    Received 27 December 2017

    Accepted for publication 12 February 2018

    Published 9 April 2018 Volume 2018:11 Pages 515—521

    DOI http://doi.org/10.2147/IDR.S160899

    Checked for plagiarism Yes

    Review by Single-blind

    Peer reviewers approved by Dr Cristina Weinberg

    Peer reviewer comments 3

    Editor who approved publication: Dr Sahil Khanna

    Objective: The relationships between fluoroquinolone and aminoglycoside resistance and single-nucleotide polymorphisms (SNPs) in gyrA , gyrB , and rpsL  genes were investigated in 95 clinical isolates of Mycobacterium avium  from China. 
    Methods: Fluoroquinolone and aminoglycoside resistance were determined by the broth microdilution method. GyrA , gyrB , and rpsL  were sequenced, SNPs were identified, and the corresponding amino acid mutations were recorded.
    Results: The M. avium  isolates displayed high levels of ofloxacin (93.68%), ciprofloxacin (92.63%), and streptomycin (65.26%) resistance. Moxifloxacin (18.95%) and amikacin (2.11%) were highly active against the strains. Fluoroquinolone resistance involving gyrA  and gyrB  gene mutations was identified. For gyrA , the most frequent SNPs were T    →C (71/95, 74.74%), followed by A→G (64/95, 67.37%) and T→C (62/95, 65.26%). The amino acid mutations occurred mainly at Gly2444Asp (GGT→GAT) (20/95, 21.05%), Ala2445Ser (GCC→TCC) (20/95, 21.05%), Ala2447Val (GCC→GTC) (20/95, 21.05%), Val2449Ile (GTC→ATC) (20/95, 21.05%), and Glu2450Gln (GAA→CAA) (20/95, 21.05%). Prominent SNPs in gyrB  included A→C (69/95, 72.63%), C→T (51/95, 53.68%), and T→G (29/95, 30.53%), and their amino acid substitutions were Ile2160Val (ATT→GTT) (21/95, 22.11%), Ile2160Met (ATT→ATG) (20/95, 21.05%), and Ile2273Leu (ATC→CTC) (11/95, 11.58%). Among the strains with aminoglycoside resistance, SNPs in rpsL  were identified mostly at position G→A (73/95, 76.84%). G→C (21/95, 22.11%) was commonly seen. The amino acid mutations primarily involved Ala1539985Thr (GCC→ACC) (19/95, 20.00%), His1539992Asp (CAC→GAC) (19/95, 20.00%), and Gln1539983Glu (CAG→GAG) (18/95, 18.95%).
    Conclusion: Our study provides valuable information that could be used for the future diagnosis and treatment of M. avium  disease.
    Keywords: Mycobacterium avium , drug resistance, single-nucleotide polymorphism, amino acid mutation, minimum inhibitory concentration


     

    摘要视频链接：SNPs related to fluoroquinolone and aminoglycoside resistance



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