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已发表论文

人体宫颈鳞状细胞癌中自噬相关标志物 Beclin1，LC3 和表皮生长因子受体 (EGFR) 的表达及临床意义

 

Authors Hu YF, Lei X, Zhang HY, Ma JW, Yang WW, Chen ML, Cui J, Zhao H

Received 18 April 2015
Accepted for publication 16 June 2015
Published 24 August 2015 Volume 2015:8 Pages 2243—2249
DOI http://dx.doi.org/10.2147/OTT.S86844
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 3
Editor who approved publication:  Professor Daniele Santini


Purpose: We aimed to investigate the expression of EGFR and the autophagy-related markers Beclin1 and LC3 in cervical cancer.
Methods: Beclin1, LC3, and EGFR expression were analyzed in 80 samples of cervical squamous cell carcinoma (SCC), 40 samples of high-grade cervical intraepithelial neoplasia (CIN), and40 samples of normal cervical tissues by immunohistochemistry. The protein expression rates were analyzed with χ 2 and Fisher’s exact tests. Differences in overall survival (OS) were determined using the Kaplan–Meier method and log-rank tests.
Results: Cervical cancer, high-grade CIN, and normal cervical epithelial cells expressed Beclin1 in 26.2%, 77.5%, and 82.5% of patients, respectively, and expressed LC3 in 28.8%, 70.0%, and 75.0% of patients, respectively. There was a significant difference between cervical SCC and high-grade CIN or normal cervical epithelial cells (P =0.000). Cervical cancer cells, high-grade CIN cells, and normal cervical epithelial cells expressed EGFR in 68.8%, 62.5%, and 12.5% of patients, respectively. There was a significant difference between cervical SCC or high-grade CIN and normal cervical epithelial cells (P =0.000). No significant association between Beclin1 or LC3 or EGFR expression and various clinicopathological parameters was observed in cervical SCC. There was no significant correlation between Beclin1, LC3, EGFR expression, and 5-year OS rates of cervical SCC patients. Beclin1- or LC3-negativity with EGFR-positivity in cervical SCC was associated with a higher Federation International of Gynecology and Obstetrics (FIGO) stage (P =0.011 and P =0.013, respectively) and pelvic lymph node metastasis (P =0.036 and P =0.092, respectively). The 5-year OS rates did not significantly differ between Beclin1- or LC3-positive and -negative patients with positive EGFR.
Conclusion: Autophagy was downregulated and EGFR was upregulated in cervical SCC. Autophagy downregulation combined with EGFR upregulation promotes the progression of cervical SCC.
Keywords: autophagy, Beclin1, LC3, EGFR, cervical squamous cell carcinoma, immuno­histochemistry


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