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已发表论文
在分子翻译和等温链置换聚合反应基础上对胶质细胞源性神经营养因子的视觉检测
Authors Zhang LY, Xing T, Du LX, Li QM, Liu WD, Wang JY, Cai J
Published Date March 2015 Volume 2015:9 Pages 1889—1896
DOI http://dx.doi.org/10.2147/DDDT.S76192
Received 21 October 2014, Accepted 3 December 2014, Published 31 March 2015
Background: Glial cell line-derived neurotrophic factor (GDNF) is a small protein
that potently promotes the survival of many types of neurons. Detection of GDNF
is vital to monitoring the survival of sympathetic and sensory neurons.
However, the specific method for GDNF detection is also un-discovered. The
purpose of this study is to explore the method for protein detection of GDNF.
Methods: A novel visual detection method based on a molecular translator and isothermal strand-displacement polymerization reaction (ISDPR) has been proposed for the detection of GDNF. In this study, a molecular translator was employed to convert the input protein to output deoxyribonucleic acid signal, which was further amplified by ISDPR. The product of ISDPR was detected by a lateral flow biosensor within 30 minutes.
Results: This novel visual detection method based on a molecular translator and ISDPR has very high sensitivity and selectivity, with a dynamic response ranging from 1 pg/mL to 10 ng/mL, and the detection limit was 1 pg/mL of GDNF.
Conclusion: This novel visual detection method exhibits high sensitivity and selectivity, which is very simple and universal for GDNF detection to help disease therapy in clinical practice.
Keywords: lateral flow biosensor, molecular translator, isothermal strand-displacement polymerization reaction
Methods: A novel visual detection method based on a molecular translator and isothermal strand-displacement polymerization reaction (ISDPR) has been proposed for the detection of GDNF. In this study, a molecular translator was employed to convert the input protein to output deoxyribonucleic acid signal, which was further amplified by ISDPR. The product of ISDPR was detected by a lateral flow biosensor within 30 minutes.
Results: This novel visual detection method based on a molecular translator and ISDPR has very high sensitivity and selectivity, with a dynamic response ranging from 1 pg/mL to 10 ng/mL, and the detection limit was 1 pg/mL of GDNF.
Conclusion: This novel visual detection method exhibits high sensitivity and selectivity, which is very simple and universal for GDNF detection to help disease therapy in clinical practice.
Keywords: lateral flow biosensor, molecular translator, isothermal strand-displacement polymerization reaction
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