Shan-Shan Li,1,2,* Jing-Ru Zhou,3,* Chun-Sheng Wang,1,2 Jia-Ling Wang,1 Yu-Qian Li,1,2 Xilizati Kulaixi,1 Li-Wei Cao,1,2 Bayina Batesurong,1,2 Jian-Rong Ye1 

1Department of Anesthesiology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, 830054, People’s Republic of China; 2The First Clinical Medical College of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, 830054, People’s Republic of China; 3Department of Anesthesiology, The Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, 830063, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Jian-Rong Ye, Department of Anesthesiology, The First Affiliated Hospital of Xinjiang Medical University, No. 137, Liyushan South Road, Urumqi, Xinjiang Uygur Autonomous Region, 830054, People’s Republic of China, Tel +86 18999938777, Email ywjianrongym@126.com

Objective: This study aims to investigate the anti-inflammatory regulatory function of signal transducer and activator of transcription 3 (STAT3) phosphorylation in hypersensitivity responses triggered by Echinococcus granulosus hydatid cyst fluid through in vitro RBL-2H3 cell culture.
Methods: RBL-2H3 cells were cultured in vitro and sensitized with immunoglobulin E (IgE), followed by intervention with STAT3 inhibitors Stattic and JSI-124. Cells were subsequently exposed to crude Echinococcus granulosus hydatid cyst fluid to induce an allergic reaction. β-Hexosaminidase (HEX) release in the cell supernatant was measured to evaluate degranulation. Apoptosis was detected using flow cytometry, and changes in phosphorylated protein levels were determined via Western Blot analysis.
Results: Analysis of β-HEX release in the Echinococcus-induced IgE-mediated RBL-2H3 cell degranulation model revealed that both inhibitors effectively inhibited mast cell degranulation (P < 0.01). Apoptosis assays revealed that both inhibitors caused varying degrees of cell damage (P < 0.01), potentially leading to late-stage apoptosis.
Conclusion: Immunoblotting analyses confirmed that treatment with the two inhibitors reduced STAT3 phosphorylation levels at the S727 and Y705 sites, thereby inhibiting cell degranulation and alleviating immune responses.

Keywords: allergic reaction, Echinococcus granulosus hydatid cyst fluid, RBL-2H3 cells, STAT3 phosphorylation